In this study, performance and exhaust emissions of a diesel engine fueled with Fuel A (60% diesel-30% biodiesel-5% ethanol-5% butanol) and Fuel B (40% diesel-50% biodiesel-5% ethanol-5% butanol) were investigated. The biodiesel produced from trap grease was obtained with an oil separator. Fuel A and Fuel B were tested in a single cylinder, four-stroke diesel engine at full load conditions. Compared with diesel fuel, the performance characteristics of blend fuels slightly deteriorated while the emission characteristics improved significantly. CO and HC emissions decreased by 87.01 and 87.50%, respectively.