The aim of this study was to estimate the total cost ofNeospora caninuminfection in cows in Turkey. According to present literature, the mean seroprevalence of infection was estimated as 14.7%. The seropositivity rate ofN. caninumin aborted cows was found to be 18%. The financial costs were estimated in US$ at 2019 prices. The total cost ofN. caninuminfection was estimated at US$710 (438-1043) per a dairy cow and the annual economic costs were estimated at US$40.5 (24.6-60.3) million for Turkey. In this study, the distribution of economic costs caused by neosporosis per cow was determined to be 67.3% for abortion, 16.8% prolonged calving interval, 4.6% milk loss, 3.5% additional artificial insemination, and 7.7% veterinary and diagnostic costs, respectively. All studies that have been conducted so far on Neosporosis are focused on the prevalence and the diagnosis of the disease in Turkey. In this study, we firstly determined the economic costs due toN. caninuminfections and try to attract attention on the economic impacts of the disease. As a result, awareness among breeders regarding the epidemiology, control, and eradication of neosporosis should be raised through training activities; coordination between the relevant institutions and organizations should be ensured; emergency action plans should be prepared; and further spread of the disease should be prevented.