In this study, the spider fauna was investigated in olive groves of Adana, Gaziantep, Hatay, Kahramanmaras, Kilis, Mersin and Osmaniye provinces during 2008-2010. The spider samples were collected using Steiner funnel, hand aspirator and by picked twigs. A total of 1055 specimens were collected consisting of the 699 juveniles and 356 adults identified. The majority of specimens belonged to the Theridiidae family with 291 individuals, followed by Thomisidae with 227 and Salticidae with 187. Collected samples consisted of 66.2% juveniles, 9.7% males and 24.1% females. The sex and the ratios of developmental stages (juvenile and adult) of each family were listed. The female/male ratio was found to be 1:2.4. The 121 spider species were recorded as belonging to 23 families from 94 genera. The dominant families regarding species richness and abundance were identified as 29 species from Salticidae, followed 22 from Thomisidae and 15 from Theridiidae. Among them, Thomisus sp. and Theridion sp. were found to be the most abundant in 24 locations and identified on trees for 9 and 10 months, respectively.