The susceptibility patterns to 16 different antibiotics of Aeromonas spp. and Pseudomonas aeruginosa isolated from Azapli and Golbasi Lakes, Adiyaman, Turkey were investigated. Totally 248 bacteria were isolated. Most of these isolates were Aeromonas sobria (35.8%) followed by Aeromonas sahnonicida (28.6%). A high percentage of Aeromonas spp. isolates from all of the sites showed resistance to cefazolin (ranged between 86.6% and 93.6%) and penicillin (ranged between 93.3% and 96.3%). Among Pseudomonas aeruginosa isolates, there was a high incidence of resistance to cefazolin (93.9% and 100.0%) and penicillin (96.9% and 100.0%, respectively). Multiple antibiotic resistance (MAR) index values ranged from 0.2 to 1 for Aeromonas spp. and Pseudomonas aeruginosa isolates. Results indicated that multiple antibiotic resistant Aeromonas spp. and Pseudomonas aeruginosa were widespread and can easily recovered from Golbasi and Azapli Lake. Contamination of water sources with pathogens, resistant to antibiotics, can cause serious diseases to humans, who are dependent on water.