This study aimed to determine the optimum autolysis conditions for the production of yeast extract. which is used to give a meaty flavor to food products and to increase their nutritional value. Autolysis was induced by incubating baker's yeast cell suspensions at different temperatures (45, 50, 55, and 60 degrees C) with a reaction time ranging from 8 to 72 h. Content and yield of total solids. alpha-amino nitrogen (alpha-AN), and protein were determined. Yeast extract powder was obtained by drying liquid yeast extract in a pilot scale spray drier. Sensory analysis was performed by adding the yeast extract powder at different concentrations (0.5%, 1.0%, 1.5%, and 2.0%) to vegetable soup. Optimum temperature and time for the production of yeast extract was 50 degrees C for 24 In, based on alpha-AN content, which was 3.7%. Furthermore, under the same conditions. protein content was 52.5% and total solids content was 1.98%. According to sensory analysis. the sample with 0.5% yeast extract powder and the control had the highest overall acceptance. According to variance analysis, differences between total solids, alpha-AN, and protein content determined at 4 different temperatures, and interactions between duration and temperature were statistically significant (P < 0.01).