DETERMINATION OF HUMAN PAPILLOMA VIRUSES DNA AND GENOTYPES IN GENITAL SAMPLES WITH PCR


Creative Commons License

Duran A. C., Erdin B. N., SAYINER A. A.

JOURNAL OF CLINICAL AND ANALYTICAL MEDICINE, cilt.8, sa.4, ss.302-306, 2017 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 4
  • Basım Tarihi: 2017
  • Doi Numarası: 10.4328/jcam.4881
  • Dergi Adı: JOURNAL OF CLINICAL AND ANALYTICAL MEDICINE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, EMBASE
  • Sayfa Sayıları: ss.302-306
  • Çukurova Üniversitesi Adresli: Evet

Özet

Aim: It is reported that each year HPV causes 528,000 new cases and 266,000 cervical cancer induced deaths worldwide. HPV16 is responsible for 54.4%, HPV18 is responsible for 16.5% of the infections. In this study, the prevalence of genital HPV infection and distribution of genotypes were investigated in women who admitted to University Hospital. Material and Method: Genital samples of 261 women were investigated for the high-risk 12 HPV genotypes (HPV16/18/31/33/35/39/45/51/52/56/58/59) with multiplex realtime PCR (Sacace Biotechnologies, Italy). Results: In 100 of 261 (38.3%) genital samples, HPV DNA were positive and all genotypes that can be detected by the kit were identified at least once. In 61 samples (61.0%) a single HPV type was identified and in 39 samples (39.0%) more than one genotype was identified. Most mixed infections (53.8%), occurred with two HPV types. In HPV DNA positive samples, the most common type was HPV16 (39/100,39.0%) followed by HPV51 (22/100,22.0%), HPV56 (18/100,18.0%), HPV52 (15/100,15.0%) and HPV31 (15/100,15.0%). The most common genotypes identified in samples infected with a single type were HPV16 (16/61,26.2%), HPV51 (9/61,14.8%) and HPV56 (8/61,13.1%). In mixed infections, HPV16 (23/39,59.0%) was followed by HPV51 (13/39,33.3%) and HPV31 (11/39,28.2%). Discussion: As a conclusion, the prevalence of genital HPV infection in the studied population of women was 38.3%. HPV16 was the most common type similar to the worldwide data, while HPV18 was less prevalent than the other high-risk HPV types, HPV51 and HPV56. Such epidemiologic studies are useful to guide development of cervical cancer screening algorithms and vaccination studies.