In vitro ovule and ovarium culture in watermelon

Gursoy I., Solmaz I., Deliboran S., Sari N.

10th EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae, Antalya, Turkey, 15 - 18 October 2012, pp.799-804 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume:
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.799-804


Unfertilizated ovaries of Halep karasi watermelon genotype were picked on the day of anthesis, one day before anthesis and one day after anthesis. Ovaries were sliced for ovary culture and ovules were extracted from ovaries for ovule culture in sterile conditions. Ovary explants and ovules were cultured on two different (MS and CBM) mediums supplemented with 1 mg/l TDZ (thidiazuron), 1 mg/l SPM (spermin) and 1 mg/l TDZ + 1 mg/l SPM. Ovary explants and ovules were incubated at 25-26 degrees C, having 3000-4000 lux light density, possessing 16/8 h light/dark photoperiod for 8 weeks. After plant regeneration from ovules and ovaries they were transferred to growth regulator free MS and CBM medium.