The purpsose of the present study was to evaluate of diagnostic and prognostic importance of the D (-) and L (+) lactic acid and some biochemical and hematological parameters of cattle with abomasal displacement. In this study, 10 cattle with right abomasal displacement, 10 cattle with left abomasal displacement and 10 healthy (control) cattle, totally 30 dairy cattle in different groups were used. Mean blood pH, HCO3- and SO2, values (P<0.01), mean value of BE (P<0.001); mean value of pvCO(2) and Cl- (P<0.05) were found statistically significant. Higher D (-) and L (+) lactic acid levels in cattle with right abomasal displacement, and higher L (+) lactic acid levels in cattle with left abomasal displacement were observed and statistically significant differences were found than healthy cattle (P<0.001). Statistically significant differences were observed between groups at mean values of GGT, ALP, LDH and AST levels (P<0.05). Mean values of total leukocytes, hematocrit and hemoglobin levels between groups were statistically significant (P<0.05). As a result, L+LA levels in blood was increased, because of the fatty liver and impaired lactate metabolism in cattle with left abomasal displacement and both D and L+LA levels increased in cattle with right abomasal displacement due to abomasal ischemia, necrosis and impaired intestinal defense, and 3 cattle died. For that reason, D (-) and (L+) lactic acid levels may be prognostic criterion in cattle with right abomasal displacement at preoperative period.