In this study it is intended to investigate the effects of yarn spinning technology on denim fabric performance. For this aim five different 100% Cotton Ne 16/1 yarn samples were produced with different spinning technologies namely; combed ring, compact, sirospun, Open-End (OE) rotor and vortex. Then denim fabric samples were woven by using these samples yarns only in weft direction. Breaking strength, tear strength, abrasion resistance, stiffness and stretch properties of denim fabric samples were tested. In order to understand the statistical importance of the spinning technology on denim fabric performance, one-way analysis of variance (ANOVA) was performed. Consequently, it is seen that yarns produced from different spinning technologies affect the woven fabric performance, significantly.