Together with technological developments, different methods in anatomic and morphometric studies have been started to be used. In fact, in this study, more than one method was used and the reliability of these methods was presented. This study was performed in the aim of getting 3D model with three dimensional (3D) reconstruction and photogrammetric methods obtained from multidetector computerized tomography (MDCT) images of femur of chinchilla by mimics program, comparing the data of both methods and presenting whether there was a difference between genders as well as left and right sides. For this purpose, 6 chinchillas of both sexes were used. First of all, MDCT images of animals were taken under general anaesthesia and 3D reconstruction was obtained after transferring the data to personal computer as DICOM format. After taking MDCT images, the femurs of the animals killed by the conventional methods were cleaned of muscle and fats by boiling and their 3D models was formed by using images via photogrammetric methods. The measurements were taken from the models of two methods and volume and surface area of femur were found significant at a level of p< 0.05 as a result of statistical processes. According to this, a statistically difference was not found in morphometric measurement values except volume and surface area. Moreover, while a statistical difference was not found between right and left femur of both sexes, a difference was determined between sexes and it was observed that the measurement values of females were higher than males. As a result, since 3D models obtained by both methods had almost same values, it was considered that these methods could be used in anatomic and morphometric studies.