Molecular characterization of Kappa-casein and beta-lactoglobulin genes in Anatolian Black cattle and Holstein breeds

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BAYRAKTAR M.

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, vol.74, no.1, pp.133-140, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 74 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.1590/1678-4162-12497
  • Journal Name: ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Page Numbers: pp.133-140
  • Keywords: kappa-Cn, beta-lactoglobulin, Anatolian Black, Holstein, polymorphism, MILK-PRODUCTION TRAITS, NATIVE CATTLE, PCR-RFLP, POLYMORPHISM, ASSOCIATION, SAHIWAL, CSN3, COWS, SEASONALITY, YIELD
  • Çukurova University Affiliated: Yes

Abstract

kappa-Cn and beta-lactoglobulin are important candidate genes associated with milk yield and milk protein content. The present investigation is carried out to determine the polymorphisms status of kappa-Cn and beta-lactoglobulin genes in Anatolian Black cattle and Holstein breeds. PCR-RFLP technique was used to determine Kappa-Casein and beta-lactoglobulin polymorphisms in both cattle breeds. The allele frequency of Anatolian Black cattle in terms of kappa-Cn and beta-lactoglobulin genes were 0.50 (A) 0.50 (B) and 0.20 (A) 0.80 (B) respectively, whereas in Holstein were 0.29 (A) 0.71 (B) and 0.44 (A) 0.56 (B) respectively. The chi-square test showed that each cattle breed was in Hardy-Weinberg equilibrium (P>0.05).