Akademik Veri Yönetim Sistemi
ULUSLARARASI HARRAN SAĞLIK BİLİMLERİ KONGRESİ 2020, Şanlıurfa, Türkiye, 5 - 07 Haziran 2020, ss.10
The causative agent of bovine tuberculosis, Mycobacterium bovis, has the largest host
range among the Mycobacterium tuberculosis complex members and it causes disease
in other animals and humans. Based on the “Single Medicine, Single Health” concept,
the cooperation of medicine and the veterinary field is highly needed in tuberculosis
cases caused by M.bovis. Molecular epidemiological studies should be conducted to
determine the way of transmission of zoonotic tuberculosis to humans and risk
factors, to identify the dominant types between humans and animals, and to
understand phylogenetic relationships of strains in our region. In our study, we aimed
to characterize 4 M.bovis isolated from the clinical samples of the patients who
applied to the hospitals of our region18 M.bovis isolated from lung, bronchial and
mediastinal lymph nodules showing granulomatous lesions in cattle in Adana
province slaughterhouses. It was determined that 22 samples were distributed in 10
clusters by MIRU-VNTR method. When the discrimination power of the primers
determining the MIRU-VNTR loci was calculated, the highest ones were determined
as ETRA, Qub26, Qub11b, Mtub21, and Miru27. When the clonal group distribution
of the isolate was examined by the spoligotyping method, a total of four spoligotype
patterns were observed. The spoligotypes determined in cattle are SB0120 / SIT482
with 9 isolates, SB0288 / SIT685 with 7 isolates, and SB0140 / SIT683. When the
spoligotypes of human isolates were examined, it was determined that 2 isolates were
SB0989 / SIT1118 and the other 2 isolates were SB0140 / SIT683. When the
similarities between the MIRU-VNTR locus patterns of human and bovine isolates
were evaluated, the presence of a 100% compatible pattern was not detected, but
some human samples were found to be 91,6% similar to a bovine sample. In
spoligotyping, 2 of the human isolates have the same spoligotype pattern as 7 cattle
isolates. In conclusion, the clonal similarity between bovine and human isolates was
determined using MIRU-VNTR and Spoligotyping methods, and it has been shown
that bovine isolates may be associated with pulmonary tuberculosis in humans.