THE CLONAL RELATIONSHIP ANALYSIS OF Mycobacterium bovis ISOLATED FROM POSTMORTEM RUMINANT LYMPH BIOPSY MATERIALS AND HUMAN CLINICAL SAMPLES BY SPOLİGOTYPİNG AND 24-LOCUS MIRU-VNTR METHODS


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Güven Gökmen T. , Yakıcı G. , Turut N., Köksal F.

ULUSLARARASI HARRAN SAĞLIK BİLİMLERİ KONGRESİ 2020, Şanlıurfa, Türkiye, 5 - 07 Haziran 2020, ss.10

  • Basıldığı Şehir: Şanlıurfa
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayısı: ss.10

Özet

The causative agent of bovine tuberculosis, Mycobacterium bovis, has the largest host range among the Mycobacterium tuberculosis complex members and it causes disease in other animals and humans. Based on the “Single Medicine, Single Health” concept, the cooperation of medicine and the veterinary field is highly needed in tuberculosis cases caused by M.bovis. Molecular epidemiological studies should be conducted to determine the way of transmission of zoonotic tuberculosis to humans and risk factors, to identify the dominant types between humans and animals, and to understand phylogenetic relationships of strains in our region. In our study, we aimed to characterize 4 M.bovis isolated from the clinical samples of the patients who applied to the hospitals of our region18 M.bovis isolated from lung, bronchial and mediastinal lymph nodules showing granulomatous lesions in cattle in Adana province slaughterhouses. It was determined that 22 samples were distributed in 10 clusters by MIRU-VNTR method. When the discrimination power of the primers determining the MIRU-VNTR loci was calculated, the highest ones were determined as ETRA, Qub26, Qub11b, Mtub21, and Miru27. When the clonal group distribution of the isolate was examined by the spoligotyping method, a total of four spoligotype patterns were observed. The spoligotypes determined in cattle are SB0120 / SIT482 with 9 isolates, SB0288 / SIT685 with 7 isolates, and SB0140 / SIT683. When the spoligotypes of human isolates were examined, it was determined that 2 isolates were SB0989 / SIT1118 and the other 2 isolates were SB0140 / SIT683. When the similarities between the MIRU-VNTR locus patterns of human and bovine isolates were evaluated, the presence of a 100% compatible pattern was not detected, but some human samples were found to be 91,6% similar to a bovine sample. In spoligotyping, 2 of the human isolates have the same spoligotype pattern as 7 cattle isolates. In conclusion, the clonal similarity between bovine and human isolates was determined using MIRU-VNTR and Spoligotyping methods, and it has been shown that bovine isolates may be associated with pulmonary tuberculosis in humans.