Chromosome and Molecular Analyses Reveal Significant Karyotype Diversity and Provide New Evidence on the Origin of Aegilops columnaris
PLANTS-BASEL, cilt.10, sa.5, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 10 Sayı: 5
- Basım Tarihi: 2021
- Doi Numarası: 10.3390/plants10050956
- Dergi Adı: PLANTS-BASEL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
- Anahtar Kelimeler: Aegilops columnaris, Ae. neglecta, C-banding, FISH, gliadin electrophoresis, sequencing, spacer regions of the chloroplast DNA, plastogroups, evolution, IN-SITU HYBRIDIZATION, GENOME DIFFERENTIATION, GENETIC DIVERSITY, AE.-COLUMNARIS, DNA-SEQUENCES, U-GENOME, TRITICUM, WHEAT, EVOLUTION, LINES
- Çukurova Üniversitesi Adresli: Evet
Özet
Aegilops columnaris Zhuk. is tetraploid grass species (2n = 4x = 28, (UUXXC)-U-C-X-C-X-C) closely related to Ae. neglecta and growing in Western Asia and a western part of the Fertile Crescent. Genetic diversity of Ae. columnaris was assessed using C-banding, FISH, nuclear and chloroplast (cp) DNA analyses, and gliadin electrophoresis. Cytogenetically Ae. columnaris was subdivided into two groups, C-I and C-II, showing different karyotype structure, C-banding, and FISH patterns. C-I group was more similar to Ae. neglecta. All types of markers revealed significant heterogeneity in C-II group, although group C-I was also polymorphic. Two chromosomal groups were consistent with plastogroups identified in a current study based on sequencing of three chloroplast intergenic spacer regions. The similarity of group C-I of Ae. columnaris with Ae. neglecta and their distinctness from C-II indicate that divergence of the C-I group was associated with minor genome modifications. Group C-II could emerge from C-I relatively recently, probably due to introgression from another Aegilops species followed by a reorganization of the parental genomes. Most C-II accessions were collected from a very narrow geographic region, and they might originate from a common ancestor. We suggest that the C-II group is at the initial stage of species divergence and undergoing an extensive speciation process.