The objective of this study was to investigate the effects of emamectin benzoate (EB) at different recommended field doses (RFD, RFDx2, RFDx4) on soil carbon mineralisation (C-m). Soils were humidified at their 40, 60 and 80% of field capacities (FC40, FC60 and FC80) and were incubated at 28 and at 32 degrees C for 45 days. All doses of EB under all temperatures and moistures significantly decreased C-m with the exception of between control and all treatments at 32 degrees C under FC40 (P < 0.05). On the other hand, increase in temperature (28 < 32 degrees C) and soil moisture (FC40 < FC60 < FC80) significantly enhanced C-m (P < 0.05). Addition of EB doses decreased potentially mineralisable carbon (C-0) at 28 degrees C but they generally enhanced at 32 degrees C. Mineralisation constant values (k) were in a narrow range from a minimum of 0.025 for RFD under FC40 at 28 degrees C to a maximum of 0.037 for control under FC40 at 32 degrees C. It is possible to conclude that all doses of EB negatively affected soil microbial activity while increase in temperature and moisture positively increased C-m.