Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry


Maraval I., Sen K., Agrebi A., Menut C., Morere A., Boulanger R., et al.

ANALYTICA CHIMICA ACTA, cilt.675, ss.148-155, 2010 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 675 Konu: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.aca.2010.07.024
  • Dergi Adı: ANALYTICA CHIMICA ACTA
  • Sayfa Sayısı: ss.148-155

Özet

A new and convenient synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor compound in rice, and its ring-deuterated analog, 2-acetyl-1-d(2)-pyrroline (2AP-d(2)), was reported. A stable isotope dilution assay (SIDA), involving headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-positive chemical ionization-ion trap-tandem mass spectrometry (GC-PCI-IT-MS-MS), was developed for 2AP quantification. A divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was used for HS-SPME procedure and parameters affecting analytes recovery, such as extraction time and temperature, pH and salt, were studied. The repeatability of the method (n = 10) expressed as relative standard deviation (RSD) was 11.6%. A good linearity was observed from 5.9 to 779 ng of 2AP (r(2) = 0.9989). Limits of detection (LOD) and quantification (LOQ) for 2AP were 0.1 and 0.4 ngg(-1) of rice, respectively. The recovery of spiked 2AP from rice matrix was almost complete. The developed method was applied to the quantification of 2AP in aerial parts and grains of scented and non-scented rice cultivars. (C) 2010 Elsevier B.V. All rights reserved.