Investigation of the Effects of Silicon Dioxide Nanoparticles and Environmental Contaminants on Immunocytotoxic and Antioxidant Defense Systems in Model Organism Galleria mellonella L.

Tuncsoy B., Idikut M., TUNÇSOY M.

Biological Trace Element Research, 2025 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s12011-025-04723-w
  • Dergi Adı: Biological Trace Element Research
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, Pollution Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Abamectin, Cadmium sulfate (CdSO4), G. mellonella, Silicon dioxide nanoparticle (SiO2 NPs)
  • Çukurova Üniversitesi Adresli: Evet

Özet

Silicon dioxide (SiO2) nanoparticles are chemically stable, biocompatible, abundant, and inexpensive. Moreover, they are highly surface-reactive. While these properties make them suitable for environmental applications, they also raise questions about their reactivity with environmental contaminants. This study investigated the enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), cytochrome P450 (Cyt P450), glutathione S-transferase (GST), and acetylcholinesterase (AChE), as well as total differential hemocyte counts and apoptotic index in the hemolymph, midgut, and fat body of Galleria mellonella, were investigated following exposure to an LD50 value of SiO2 NPs (396 μg/mL), an environmental concentration of abamectin, cadmium sulfate (CdSO4), and copper sulfate (CuSO4), both singly and in a mixture. A decrease in the total hemocyte count was observed in the groups that were singly treated with SiO2 and CdSO4. However, an increase was observed in the SiO2 NPs + CdSO4 mixture, and a decrease was observed in the SiO2 NPs + abamectin group compared with the control. Treatment with SiO2 NPs, CdSO4, and abamectin singly and in mixture altered the levels of prohemocytes, plasmatocytes, spherulocytes, granulocytes, and oenocytoids. This study determined that SiO2 NPs, CdSO4, and abamectin lead to toxic effects in G. mellonella larvae following single and mixture applications. It was also observed that SiO2 NPs may increase the toxic effects of environmental pollutants on the antioxidant defense and immune systems, depending on tissue differences following mixture applications.