Nucleotide sequence of CVd-Ib and CVd-IV viroids collected from citrus gummy bark-diseased sweet orange trees in the east Mediterranean region of Turkey


Onelge N.

ZEITSCHRIFT FUR PFLANZENKRANKHEITEN UND PFLANZENSCHUTZ-JOURNAL OF PLANT DISEASES AND PROTECTION, cilt.103, ss.482-487, 1996 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 103 Konu: 5
  • Basım Tarihi: 1996
  • Dergi Adı: ZEITSCHRIFT FUR PFLANZENKRANKHEITEN UND PFLANZENSCHUTZ-JOURNAL OF PLANT DISEASES AND PROTECTION
  • Sayfa Sayıları: ss.482-487

Özet

Two citrus viroids CVd-Ib and CVd-IV, collected from citrus gummy bark-diseased sweet orange trees in Turkey, were amplified using RT-PCR and directly sequenced from gel-purified products by the Promega fmol sequencing system. CVd-Ib, a member of the apple scar skin viroid (ASSVd) family, was determined 317 nucleotides in length and CVd-IV, a natural chimeric viroid of citrus exocortis viroid (CEVd) was found 285 nts in length. The obtained nucleotide sequences of both viroids were compared with the type strains of CV-Ib and CVd-IV previously sequenced from avocado and grapefruit in Israel.