Box-Behnken design based optimization of phenolic extractions from Polygonum equisetiforme roots linked to its antioxidant and antibacterial efficiencies

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Elgudayem F., Aldiyab A., Albalawi M. A., Omran A., Kafkas N. E., Saghrouchni H., ...Daha Fazla

Frontiers in Sustainable Food Systems, cilt.7, sa.1, ss.1-14, 2023 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.3389/fsufs.2023.1141207
  • Dergi Adı: Frontiers in Sustainable Food Systems
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.1-14
  • Çukurova Üniversitesi Adresli: Evet

Özet

Purpose: The Polygonum equisetifome is a prospective plant source of high

protein, unsaturated fatty acids, and useful safe bioactive molecules. Therefore,

the aim of this study was to optimize the ultrasonic aqueous extraction of phenols

from P. equisetifome roots using Box-Behnken design based statistical modeling,

and to evaluate the antioxidant and antibacterial eciencies of P. equisetifome

root extracts against pathogenic bacteria.

Methods: In this study, the box-behnken design was used to optimize

the extraction of phenols. The extraction temperature (30–70◦C), ultrasound

assisted extraction (UAE) time (1–9min), and liquid-solid ratio (35–45mL/g) were

investigated as the factors that influence the phenolic yield (Y1) and their DPPH

(1,1-diphenyl-2-picrylhydrazyl) scavenging activity (Y2).

Results: The optimal conditions for both responses were 50◦C, 5min, and

40mL/g. At these conditions, Y1 reached its maximum to be 45.321mg GAE/g

dry weight and Y2 to be 120.354 μmol Trolox/g dry weight. The P. equisetifome

roots contained water soluble phenol, high anthocyanin, and condensed tannins.

Interestingly, the P. equisetifome extracts showed a relation to its antioxidant

and antibacterial activities, FRAP (Ferric-reducing/antioxidant power), and ABTS

scavenging activity were determined. The morphological and physico-chemical

features of the extract were analyzed using SEM-EDX, FT-IR, and minimum

inhibitory concentration (MIC) was analyzed against several pathogenic bacteria.

The antibacterial activity of the extract showed that the extract is more ecient

against Staphylococcus aureus, while the P. equisetifome extracts showed ecient

MIC against S. aureus, followed by Bacillus cereus.Suggestions: The relation of P. equisetifome extracts to its antioxidant, and

antibacterial eciencies open a new avenue of their potential uses in the food

and pharmaceutical industries.

KEYWORDS

biological activities, Box-Behnken design, chemical characterization, P. equisetiforme

roots, water-soluble phenolic compounds, ultrasonic-assisted extraction