The absolute essential oils obtained from the bulbs and the aerial parts of M. neglectum collected from Kahramanmaras (Turkey) were analysed by GC-FID and GC-MS. In GC-MS analyses, 17 components comprising 100% of the essential oil of the bulbs and 17 components comprising 97.1% of the essential oil of the aerial parts. Major constituents of the absolute essential oil of the bulbs were found to be ethyl palmitate (18.7%), ethyl linoleate (16.3%), palmitic acid (15.2%) and linoleic acid (10.8%). The aerial parts oil mainly contents palmitic acid (28.5%), oleic acid (10.9%), linoleic acid (22.6%) and linolenic acid (11.4%). The absolute essential oils were analyzed by using microdilution assay for antimicrobial activity against three Gram-positive, four Gram-negative bacteria and one of fungus. The oils obtained from bulbs and aerial parts possessed activity against Staphylococcus epidermidis with MIC values of 625 and 1250 mu g/mL, respectively. The bulbs oil showed antibacterial activity against Enterococcus faecalis and Escherichia coli with 156.2 mu g/mL and 625 mu g/mL MIC values. The bulbs oil exhibited antifungal activity against Candida albicans with 312.5 mu g/mL MIC value. This is the first report on the chemical composition and antimicrobial activity of the essential oils obtained from M. neglectum growing in Turkey.