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Advences In Predictive & Personalized Medicine, İstanbul, Turkey, 2 - 03 April 2015, pp.54
Background: Lipid peroxidation is closely related to pathophysiological events resulting from various diseases. Reactive oxygen species which cannot be removed by antioxidant system leads to damage cells with causing lipid peroxidation. Various unsaturated or dicarboxylic aldehydes formed during lipid peroxidation are believed to cause toxicity. Sepsis, mastitis, acidosis, ketosis, enteritis, pneumonia is associated with many diseases such as respiratory and joint diseases. In our study, development of a new determination protocol for measuring of malondialdehyde (MDA) with 1,1,3,3 tetramethoxypropane using in tissue fluid, by microspectrophotometric method was aimed. Metod: For this purpose, the scale of the daily standard was reduced at microlitre rate which is measured from the dilution of the stock standard solution (1,1,3,3 tetramethoxypropane). Also the study was supported by optimization experiments to minimize the experimental / personal errors which are resulted from microspectrophotometry. Results: This study showed that the results of newly developed method are statistically highly in accord with the result of commonly used methods. (r= 0, 99; p<0,01). Conclusion: A high correlation was detected in microspectrophotometric MDA measurements between 0-5 minutes. Also it is observed that different absorbance values which are resulted from different measurement materials should be taken into account. In addition, it is concluded that by using microspectrophotometre in MDA studies, it has been found to be advantageous to study plurality of measurements in a short time and with much less tissue fluid / sample will be required. Acknowledgement: This work was supported by the Research Fund of Cukurova University. Project Number: ‘’TF-2012-BAP8”.