A case of abnormal lobulation of spleen in a three years old Holstein cow was described after slaughter. Post mortem evaluation of the cow revealed three lobes and two projections at the spleen. The color of the spleen was normal but lymphoid hyperplasia was observed at the cut surface. Hilus of the spleen was located on 1/3 of the facies visceralis. Histopathological examination of the spleen revealed that lymphoid hyperplasia. No abnormality was observed other visceral organs. This is a first case of lobulated spleen in a three years old Holstein cow..