Introduction: One of the most common mechanisms in the development of carbapenem resistance is acquiring carbapenemases. A new and different carbapenemase, Klebsiella pneumoniae carbapenemase (KPC), has recently emerged in Turkey. Klebsiella pneumoniae carbapenemases are encoded by the bla(KPC) gene and can be transferred between different species. Reports showing the presence of bla(KPC) gene in isolates from Turkey are limited. The current study aimed to investigate the presence of bla(KPC) gene in carbapenem resistant K. pneumoniae and Escherichia coli isolates in a tertiary care hospital in Turkey.