Akademik Veri Yönetim Sistemi
RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY, cilt.18, sa.10, ss.4882-4890, 2025 (Scopus)
Facts show that many Indonesians are smokers. Some of these smokers are kretek smokers. Kretek is made from a mixture of tobacco, cloves, and sauce. Kretek smoke is toxic, and affects the cerebellum, especially Purkinje cells. Aim of this study was to determine the effects of kretek smoke on the Purkinje cells of rats. CG (control group) were rats that breathed using normal air (not exposed to kretek smoke). TG (treatment group) were rats that were exposed to smoke of kretek 1 stick/day for 1 month (30 days). Observations were made on the morphology of the rat brain and histology on the cerebellum. Qualitative and quantitative observations were made on Purkinje cells (area, perimeter, length, and width). The nicotine content in the kretek cigarettes used in this study was 3.88±0.05µg/g. Exposure to kretek smoke of 1 stick/day for 1 month in rats demonstrated that the brain did not experience changes in shape, but Purkinje cells in the cerebellum became abnormal in both shape and size. Purkinje cells area of rats in CG was higher than TG (p = 0.000). Purkinje cells perimeter of rats in CG was higher than TG (p = 0.000). Purkinje cells length of rats in CG was higher than TG (p = 0.000). Purkinje cells width of rats in CG was higher than TG (p = 0.000). Exposure to kretek smoke of 1 stick/day for 1 month did not change the morphology of the rat brain, but reduced the size of Purkinje cell bodies, and changed the shape from round to tapered as a sign of cell degeneration.