Effects of sulfhydryl compounds on the inhibition of erythrocyte membrane Na+-K+ ATPase by ozone

Bilgin R., Gul S., Tukel S. S.

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, vol.47, no.2, pp.227-232, 1999 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 2
  • Publication Date: 1999
  • Journal Name: BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.227-232
  • Çukurova University Affiliated: Yes

Abstract

Exposure of human erythrocyte membranes to ozone (5 mu mol/10 min) resulted in the inhibition of erythrocyte membrane Na+-K+ ATPase (EC.3.6.1.39). It was determined that, the degree of enzyme inhibition in the directly ozone exposed membranes was greater than that of membranes obtained from ozone exposed intact erythrocytes. In the presence of varying concentrations (0-1.0 mM) of dithiotrethiol or mercaptoethanol Na+-K+ ATPase activities of both types of ozone exposed membranes were increased almost proportionally with the concentration of dithiotrethiol or mercaptoethanol however, the activities were still lower than the normal Na+-K+ ATPase value.