Characterization of ATPases in the gill of freshwater mussel (Unio tigridis) and effects of ionic and nanoparticle forms of aluminium and copper

Canli E. G., CANLI M.

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, cilt.247, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 247
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.cbpc.2021.109059
  • Dergi Adı: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Characterization, ATPase, Mussel, Aluminium, Copper, Nanoparticle, CELLS IN-VITRO, CA2+-ATPASE ACTIVITY, PLASMA-MEMBRANE, NA+/K+-ATPASE, FISH, CADMIUM, LEAD, SENSITIVITY, ZINC, EXPRESSION
  • Çukurova Üniversitesi Adresli: Evet

Özet

Mussels are often used to monitor the aquatic systems for different ecological aims, as they are one of the best bioindicator animals. However, the characterization of biomarkers should be known before using them in environmental monitoring and toxicology studies. There is no study to our knowledge on the characterization of Na-ATPase and Ca-ATPase in tissues of freshwater mussel (Unio tigridis). Thus, this study was undertaken to characterize the optimum working conditions of Na-ATPase and Ca-ATPase in the gill of mussels, determining the highest levels of parameters (Na+, Mg2+, Ca2+, ATP, pH, temperature, enzyme amount, incubation time) to obtain maximum activity. The present study also aimed to investigate in vitro effects of ionic and nanoparticle (Al2O3, CuO) forms of aluminium and copper (0, 30, 90, 270 mu g/L) on the activities of Na-ATPase and Ca-ATPase. Data showed that there was no ouabain-sensitive ATPase activity in the gill up to 10 mM ouabain concentrations. Na-ATPase and Ca-ATPase activities in the gill of control mussels were 5.124 +/- 0.373 and 3.750 +/- 0.211 mu mol Pi/mg pro./h, respectively. Exposure to different concentrations of nanoparticles did not alter significantly (P 0.05) the activities of Na-ATPase and Ca-ATPase in vitro, whereas the same concentrations of ionic aluminium and copper significantly decreased (P < 0.05) the enzyme activities. Data emphasized that there were different modes of action between ionic and nanoparticle forms of aluminium and copper. Data also suggested that in vivo studies should also be carried out to estimate better the effects of nanoparticle and ionic forms of metals on ATPases of U. tigridis.