In this study different grapevine genotypes having different resistance levels to CaCO3 were used as plant material. Genotypes were grown in pots including CaCO3 Concentrations of 10, 30 and 50 %. For each medium, 4 different soil Fe applications were performed. These are: (i) 20 ppm Fe (as FeSO4) + farmyard manure (100 g/pot/5 kg soil), (ii) 20 ppm Fe (as Fe-EDDHA) (iii) 20 ppm Fe (as FeSO4) + citric acid (as 10 % percentage of applied FeSO4), (iv) control (soil without Fe). In order to determine the effects of applications on active and total iron concentrations of leaves were examined. As a result of the study, iron concentrations of all tested genotypes were decreased with increasing levels of CaCO3. Applications of Fe-EDDHA and FeSO4 + citric acid showed better results when all applications compared in respect of leaves active and total iron concentrations.