Purpose This study determined the anxiety and hopelessness levels of women during in vitro fertilization-embryo transfer. Design and Methods This cross-sectional and descriptive study was conducted with 50 infertile women. The Personal Information Form, State-Trait Anxiety Inventory, and Beck Hopelessness Scale (BHS) were used to collect data. Findings A statistically significant difference between the BHS total score before oocyte pick-up and after embryo transfer was found (P < .05). However, no statistically significant relationship was found between state anxiety in the same process (P > .05). Practice Implications This study showed that women had high levels of hopelessness and as a woman's state anxiety level increased, her level of hope decreased.