Artificial seeds of pepper somatic embryos


Buyukalaca S. , MAVITUNA F.

1st International Symposium on Solanacea for Fresh Market, Malaga, İspanya, 28 - 31 Mart 1995, ss.106-110 identifier

  • Cilt numarası:
  • Basıldığı Şehir: Malaga
  • Basıldığı Ülke: İspanya
  • Sayfa Sayıları: ss.106-110

Özet

Artificial seeds are a potential method for delivering somatic embryos to the field. Mature somatic embryos were encapsulated in calcium alginate gel using different concentrations of sodium alginate (2-6%) and calcium chloride (25-100 mM) in order to determine the effect of rigidity of bead on the conversion frequency of somatic embryos, MS medium supplemented with 3% sucrose was used far germination. Variability was obtained between sodium alginate and calcium cloride concentration with respect to % germination. Plant regeneration frequency decreased with an increase in sodium alginate concentration above 3%. 3% sodium alginate and 75 mM calcium chloride gave the best conversion and optimum bead rigidity and shape. In vitro and in vivo conversion of naked and encapsulated embryos were compared.