Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems


Bayraktar M., Şimşek Sezer E. N.

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, cilt.5, sa.2, ss.792-799, 2021 (Hakemli Dergi)

Özet

Light is a very important factor in growth and reproduction. This study aims to determine the effect of two different lighting systems on the expression of MyoD, IGF-I and STAT5B genes in the leg muscles of the Japanese quail. For this aim, two lighting systems were used; 16 hours’ light: 8 hours’ darkness (16 h L: 8 h D) group I and 8 hours’ light: 16 hours’ darkness (8 h L: 16 h D) group II. Quantitative real-time PCR was used for assessment of the gene expression levels in leg muscles. When the Real Time PCR results were evaluated, it was determined that MyoD gene expression was higher in group I than in group II. On the other hand, when IGF-I and STAT5B gene expressions were evaluated, the opposite situation was found, namely higher expression in group II compared to group I.