Akademik Veri Yönetim Sistemi
Bioresource Technology Reports, cilt.34, 2026 (ESCI, Scopus)
The growing global demand for fish has led to increased processing waste, representing 50–80% of total biomass and remaining underutilized despite its rich protein and lipid content. This study explores the use of fish processing waste as a low-cost substrate for microbial lipase production, contributing to sustainable waste valorization. Lipase production was optimized using a multifactorial experimental design, considering the influence of pH, temperature, and substrate concentration on enzyme activity. Bacillus licheniformis was cultured under varying fish waste (5–20%) and glucose (1–5%) concentrations for 24–72 h. Lipase activity, determined by a titrimetric method, reached a maximum of 4.83 U/mL at 10–15% fish waste and 5% glucose after 48 h. ANOVA and regression analyses (R2 = 0.91) confirmed significant effects of both parameters. The enzyme exhibited stability at 35–45 °C and pH 6–8, supporting its industrial potential. Overall, fish processing waste proved to be an efficient and sustainable substrate for lipase synthesis, providing an environmentally friendly approach to converting marine by-products into valuable biotechnological materials.