Use of indirect excretory/secretory enzyme-linked immunosorbent assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from eastern Turkey

Şimşek S., Koroglu E., Utuk A. E., Altay K.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, vol.30, no.4, pp.411-415, 2006 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 4
  • Publication Date: 2006
  • Journal Name: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.411-415
  • Çukurova University Affiliated: No

Abstract

The aim of this study was to investigate fasciolosis in a group of eosinophilic and non-eosinophilic cattle in eastern Turkey. All the cattle were examined for antibodies against F. hepatica by modified indirect excretory/secretory enzyme-linked immunosorbent assay (ES-ELISA). Of the 1200 cattle tested, 164 (13.6%) were found to be eosinophilic by absolute eosinophil counts. Out of the 164 eosinophilic cattle, 121 (73.7%) were found to be positive for fasciolosis by indirect ES-ELISA, while the rate was only 35% among the non-eosinophilic group (P < 0.001). Our data indicate that parasitic infections should be considered an important cause of eosinophilia, particularly in fasciolosis, and the indirect ES-ELISA is useful as a screening test to examine anti-F. hepatica antibodies for the diagnosis of fasciolosis in cattle.