Aflatoxin M-1 (AFM(1)) is a highly toxic compound found in milk and milk products, and its presence in milk is a potential threat to the health of consumers of dairy products. For this reason biological detoxification techniques are required for the elimination of AFM(1) from contaminated products. The ability of 5 probiotic bacteria, 3 Lactobacillus and 2 Bifidobacterium strains, were studied for their AFM(1) binding ability. The strains were incubated both in phosphate-buffered saline and skim milk for 24 h at 37degreesC and the toxin residue in the supernatant was measured using thin-layer chromatography. Abilities of tested strains to bind AFM(1) were found to range from 25.7 to 32.5% for phosphate-buffered saline and 21.2 to 29.3% for skim milk.