An immunohistochemical study in cases with usual and unusual clinicopathological findings of canine visceral leishmaniosis


Toplu N. , Aydogan A.

PARASITOLOGY RESEARCH, cilt.109, ss.1051-1057, 2011 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 109 Konu: 4
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s00436-011-2345-0
  • Dergi Adı: PARASITOLOGY RESEARCH
  • Sayfa Sayısı: ss.1051-1057

Özet

The present study describes pathologic findings and immunohistochemical diagnosis of canine visceral leishmaniasis (CVL) in 22 dogs who died naturally in the Aegean region of Turkey. At necropsy, lymphadenomegaly, hepatosplenomegaly, hepatic, and nephrosclerotic lesions were conspicuous. Histopathologically, chronic inflammatory reactions of the spleen, lymph nodes, bone marrow, liver, and skin were marked findings. Cytological and histological examinations showed macrophages loaded with Leishmania amastigotes in these organs. Immunohistochemistry revealed that immunolabeling of amastigotes and/or parasite antigen, especially in the lymph nodes, spleen, bone marrow, liver, and skin, and occasionally, in the kidneys, intestines and lungs. Our laboratory results showed that immunohistochemistry should be included, along with cytological and histological examinations, in the diagnosis of CVL.