Immunohistochemical evaluation of bcl-2, ER-alpha, caspase-3,-8,-9, PCNA and Ki-67 expressions in canine mammary carcinomas


Aydogan A. , Ozmen O., Haligur M. , Sipahi C., Ileri D., Haligur A.

BIOTECHNIC & HISTOCHEMISTRY, vol.93, no.4, pp.286-292, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 93 Issue: 4
  • Publication Date: 2018
  • Doi Number: 10.1080/10520295.2018.1434680
  • Title of Journal : BIOTECHNIC & HISTOCHEMISTRY
  • Page Numbers: pp.286-292

Abstract

We investigated the expression of bcl-2, estrogen receptor alpha (ER-alpha), caspase-3, -8, -9, proliferating cell nuclear antigen (PCNA) and Ki-67 in canine mammary carcinomas. We used 65 paraffin embedded and re-diagnosed archival canine mammary tumor samples to which we applied the routine streptavidin-biotin-peroxidase technique. Seventeen cases were re-diagnosed as tubulopapillary carcinoma, 31 were re-diagnosed as complex carcinoma and 17 were re-diagnosed as carcinosarcoma. Differences of expression of bcl-2 and PCNA were statistically significant according to tumor type. Differences in expression of ER-alpha, caspase-3, -8, -9 and Ki-67 were not statistically significant. Differences of expression of bcl-2 and PCNA were statistically significant compared to ER-alpha, caspase-3, -8, -9 and Ki-67 in carcinosarcomas. We report the prognostic significance of bcl-2 and PCNA expression in canine mammary carcinosarcomas.