In the present study, effects of pH at 5.0, 7.0 and 9.0 levels on uptake of lead, content of chlorophyll and nitrogen were investigated in Polygonum salicifolium. Total chlorophyll and nitrogen contents in P. salicifolium were adversely affected by Pb2+ concentrations and toxicity of them was pH-dependent. The plant was adversely affected by pH 5.0, more than by pH 9.0. After 12-days examination of Pb2+ treatment, the results showed that lead accumulation among plant tissues was variable. The parts of the plants of P. Salicifolium revealed Pb2+ accumulation at all pH levels tested and the metal concentrations occurred in the following order: root>stem>leaf. Pb2+ concentrations in plant tissues in relation to pH were generally found in P. salicifolium in following order: in the roots: 9.0>7.0>5.0; in the stems and the leaves: 7.0>9.0>5.0.