Acoustic emission (AE) is a nondestructive testing (NDT) technique used for detecting damages, cracks, and leaks in different structures such as metals, composites, wood, fiberglass, ceramics, plastics, etc. In recent years, AE has gained popularity within the field of biomedical applications. The structure of bone is similar to composite materials, therefore, it is advantageous to use NDT technique. Thus, it can be used for monitoring the fracture behavior, crack initiation/propagation, and fatigue detection in bones. The goal of this study was to determine the usefulness of AE techniques in fracture detection phase of bones and to develop an NDT methodology for the monitoring of crack initiation and propagation in bones. This study describes AE activity during fracture of bone tissue under tensile loads. The experiments were carried out in vitro techniques using intact and fracture-simulated bovine tibias. The specimens were loaded to failure in tension using a mechanical testing machine. During the mechanical tests, AE signals were measured and recorded by using AE system processor equipped with two wideband piezoelectric sensors fixed to the surfaces of both ends of the test specimens. By superposing the load-time curve and the cumulative AE event-time curve, AE activities of crack initiation and propagation were identified. In all experiments, the cumulative AE number for each period of time rose up exponentially with the incremental tensile load. Load for AE initiation demonstrated a convincing linear interaction with AE event generation.