APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, vol.18, no.1, pp.1611-1620, 2020 (SCI-Expanded)
Myrtle is known to be an important aromatic and medicinal plant species. Myrtle plant is consumed mainly as spice having several uses in the perfumery, food and pharmaceutical industries. It is also widely used for ornamental purposes such as fencing, roadsides, and green cutting. Plant tissue culture techniques are an essential tool to micropropagate for the plant producer. TIS (Temporary immersion systems) is an alternative plant tissue culture technique for micropropagation mechanism in plant species. A new culture vessel named PlantForm TIS bioreactor has been recently developed. In the present study, we aimed to evaluate and compare a new type of TIS bioreactors with agar media for myrtle. MS (Murashige and Skoog) medium supplemented with 1 mg l(-1) BAP (benzylaminopurine) was used for multiplication, with 1 mg l(-1) IBA (indole-3-butyric acid) for rooting. Two different immersion times (4 h and 8 h) were compared with the solid agar medium. Plantlets growing in PlantForm systems performed better than those in the solid medium. All data recorded at micropropagation and rooting treatments were much more successful. In conclusion, PlantForm TIS bioreactor presents a valid alternative to traditional in vitro micropropagation and rooting systems, resulting in a reduction of cost, labor, and time for the mass propagation.