In Turkey, quince (Cydonia oblonga Mill.) is found as both a wild and a cultivated tree species. Me Karanlikdere Valley is a pit area between Sefaatli and Yerkoy districts in Yozgat Province that originates from the River Delice. Members of the Yozgat Karanlikdere Valley quince population, containing 17 quince accessions and 15 commercial quince cultivars, were DNA fingerprinted using 30 simple sequence repeat (SSR) primers to identify the genetic relationships among them. A total of 111 alleles were detected for all 32 accessions, and the number of alleles revealed by SSR analysis ranged from 2 to 10 alleles per locus with a mean value of 3.70 alleles per locus. The Ms06g03 primer gave the highest number of alleles (Na = 10). Polymorphism information content (PIC) values ranged from 0.20 (CH05e04) to 0.78 (Ms06g03) with a mean PIC value of 0.45. Structure analysis and unweighted pair group method with arithmetic average (UPGMA) clustering of the accessions depicted three major clusters, where several pairs of accessions could not be separated. This study indicated that the SSR markers could be utilized as a reliable tool for the determination of genetic variations and relationships of quince accessions. Furthermore, the results of this study will be useful for starting a cross-breeding cultivar program for quince.