INDIRECT SELECTION OF CRE1 GENE IN WINTER WHEAT POPULATIONS


Caliskan M. , Uranbey S., Nicol J., Akar T., Elekcioglu H. , Kaya G.

ARCHIVES OF BIOLOGICAL SCIENCES, cilt.63, ss.49-53, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 63 Konu: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.2298/abs1101049c
  • Dergi Adı: ARCHIVES OF BIOLOGICAL SCIENCES
  • Sayfa Sayıları: ss.49-53

Özet

The nematodes are important biotic constraint in rain-fed wheat production systems. In Turkey, they is found in 75.0% of soil samples in Central Anatolia with the dominant species being Heterodera filipjevi. Yield losses for winter wheat in rain-fed environments are documented between 27.0-46.0 %. A single dominant gene for resistance to H. avenae, designated as Cre1, was assessed in Turkey. It was also found to be effective to Heterodera filipjevi. In this research, a STS-based Cre1 marker was applied in a number of segregating wheat populations from F(1) to F(4) to discriminate Cre1-positive lines among the wheat populations. Results clearly indicated that Marker Assisted Selection (MAS) is functioning effectively, with recovery of Cre1 positive lines up to 88.0 % depending on the cross in early stage of breeding.

The nematodes are important biotic constraint in rain-fed wheat production systems. In Turkey, they is found

in 75.0% of soil samples in Central Anatolia with the dominant species being Heterodera filipjevi. Yield losses for winter wheat in rain-fed environments are documented between 27.0-46.0 %. A single dominant gene for resistance to H. avenae, designated as Cre1, was assessed in Turkey. It was also found to be effective to Heterodera filipjevi. In this research, a STSbased Cre1 marker was applied in a number of segregating wheat populations from F1 to F4 to discriminate Cre1-positive lines among the wheat populations. Results clearly indicated that Marker Assisted Selection (MAS) is functioning effectively, with recovery of Cre1 positive lines up to 88.0 % depending on the cross in early stage of breeding.

Key words: Bread wheat, nematode, Cre1 resistance, STS-PCR, indirect selection.