CONSTRUCTION OF RECOMBINANT YEAST (Saccharomyces cerevisiae) PRODUCING beta(1.3) GLUCANASE AS A FISH FEED ADDITIVES


MAZI G. , BAYLAN M. , ÖZCAN N.

SCIENTIFIC PAPERS-SERIES D-ANIMAL SCIENCE, cilt.59, ss.42-47, 2016 (ESCI İndekslerine Giren Dergi) identifier

  • Cilt numarası: 59
  • Basım Tarihi: 2016
  • Dergi Adı: SCIENTIFIC PAPERS-SERIES D-ANIMAL SCIENCE
  • Sayfa Sayıları: ss.42-47

Özet

The main goal of the feed industry is to manufacture the high quality food economically by increasing the biological value of the feed proteins. An addition to that, it is to provide higher quality animal products in sufficient amounts while keeping the input costs at optimum levels. Keeping these goals in our mind, in this study, the pRS416G recombinant vector carrying the beta(1.3) glucanase enzyme, which has indirecting effects on the immune system significantly was transferred to Saccharomyces cerevisiae using the electroporation method. PCR reaction where Saccharomyces cerevisiae/pRS416G DNA is used as a template was examined on % 0.8 agarose gel yielding approximately 1.9 kb double dagger DNA band carrying the beta(1.3) glucanase gene was visualized. After a zymogram analysis, the yellow zone expressed by beta(1.3) glucanase gene was observed in the recombinant yeast extracts. This study will be pioneer work for the development of recombinant single cell proteins, thereby including immune stimulant of the beta(1.3) glucanase as fish feed additives.