Indirect and direct energy consumption for the production of biomass, which will be used as a biofuel raw material, is very important. Therefore in this study, it is aimed to evaluate fossil fuel consumption and its associated carbon dioxide (CO2) emissions to cultivate sugar beet used as a bioethanol raw material. The total diesel consumption of the tractor with the implements/machines and harvesting machine was calculated as 1071 L per hectare (ha). The total CO2 emissions for sugar beet cultivation were calculated as 293.8 kgCO(2) per hectare. It was found that the total fossil fuel (diesel+lubricant oil), energy consumptions and CO2 emissions for sugar beet cultivation were 107.97 L/ha, 4006.6 MJ/ha and 296.5 kgCO(2)/ha, respectively. The specific value of fossil energy consumption related to total fossil fuel was 70.48 MJ per ton tuber. Taking into account the values of specific fuel consumption, 1.9 L fossil fuel was consumed to produce 1 ton sugar beet tuber. Considering the specific value of tuber production, I L fossil fuel was consumed to produce 526.57 kg sugar beet tuber. Taking into account the specific value of CO2 emission, 5.22 CO2 was released to produce 1 ton sugar beet tuber. For 1 kg ethanol production 0.15 L fossil fuel or 5.72 MJ fossil fuel energy corresponding to this amount of fuel consumption is consumed. As a result, fossil fuel consumption for the production of 1 kg of ethanol from sugar beet molasses results in an emission of 0.42 kgCO(2).