PURIFICATION OF COPPER-ZINC SUPEROXIDE DISMUTASE FROM HUMAN ERYTHROCYTES AND PARTIAL CHARACTERIZATION

KARADAG, H.; BİLGİN, RAMAZAN

doi:10.2478/v10133-010-0021-7

PURIFICATION OF COPPER-ZINC SUPEROXIDE DISMUTASE FROM HUMAN ERYTHROCYTES AND PARTIAL CHARACTERIZATION

KARADAG H., BİLGİN R.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.24, sa.1, ss.1653-1656, 2010 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 24 Sayı: 1
Basım Tarihi: 2010
Doi Numarası: 10.2478/v10133-010-0021-7
Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.1653-1656
Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
Çukurova Üniversitesi Adresli: Evet

Özet

Copper-zinc superoxide dismutase (CuZnSOD; E.C:1.15.1.1) catalyzes the dismutation of the superoxide radical to hydrogen peroxide and oxygen. In this study, CuZnSOD was isolated from human erythrocytes using DEAE-celltilose chromatography and copper chelate affinity chromatography. The enzyme was purified 196.3 fold with 33.8% efficiency. The molecular weight of CuZnSOD was determined as 20 kDa by SDS-PAGE.