PURIFICATION OF COPPER-ZINC SUPEROXIDE DISMUTASE FROM HUMAN ERYTHROCYTES AND PARTIAL CHARACTERIZATION


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KARADAG H., BİLGİN R.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.24, ss.1653-1656, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 24 Konu: 1
  • Basım Tarihi: 2010
  • Doi Numarası: 10.2478/v10133-010-0021-7
  • Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Sayfa Sayıları: ss.1653-1656

Özet

Copper-zinc superoxide dismutase (CuZnSOD; E.C:1.15.1.1) catalyzes the dismutation of the superoxide radical to hydrogen peroxide and oxygen. In this study, CuZnSOD was isolated from human erythrocytes using DEAE-celltilose chromatography and copper chelate affinity chromatography. The enzyme was purified 196.3 fold with 33.8% efficiency. The molecular weight of CuZnSOD was determined as 20 kDa by SDS-PAGE.