Akademik Veri Yönetim Sistemi
Genetic Resources and Crop Evolution, cilt.73, sa.1, 2026 (SCI-Expanded, Scopus)
Pistachio (Pistacia vera L.) is challenging to propagate due to its low regenerative capacity clonally; this study investigates the propagation potential of ‘Uzun’ and ‘Siirt’ cultivars using different media and plant growth regulators (PGRs). In surface sterilization trials with Siirt seeds, treatment with 20% sodium hypochlorite for 40 min provided the highest sterilization rate (80%) and the lowest contamination rate (20%). Germination experiments showed the highest rates in Siirt (71%) and Uzun (69%) cultivars on MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP), 0.1 mg/L indole-3-butyric acid (IBA), and 0.25 mg/L gibberellic acid (GA₃). In shoot proliferation, MS medium outperformed woody plant medium (WPM), with Siirt showing the highest shoot number (2.6) and length (3.2 cm) using the same hormone combination. Cytokinin dose trials also confirmed the superiority of 1.0 mg/L BAP, resulting in an average of 2.6 shoots per explant in both cultivars. The average shoot lengths were 2.6 cm for Siirt and 2.2 cm for Uzun. Although Siirt generally showed better performance, differences between cultivars were not statistically significant. For rooting, the best response was observed in ½ MS medium supplemented with 1 mg/L each of IBA, indole-3-acetic acid (IAA), and 1-naphthaleneacetic acid (NAA), and 1 g/L activated charcoal. Under these conditions, Siirt had the highest average root number (3.20), while Uzun produced the longest roots (2.67 cm). The same medium achieved the best rooting performance overall, resulting in average root lengths of 2.53 cm for Siirt and 2.67 cm for Uzun. These results indicate that MS-based media and optimized PGRs improve in vitro propagation of pistachio cultivars and provide a tool for conserving genetic resources and agrobiodiversity.