Molecular typing and antifungal susceptibility profile of Candida krusei bloodstream isolates from Türkiye

Karakoyun A. S., Spruijtenburg B., Unal N., Meijer E. F. J., SUCU M., Hilmioǧlu-Polat S., ...Daha Fazla

Medical Mycology, cilt.62, sa.2, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 2
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1093/mmy/myae005
  • Dergi Adı: Medical Mycology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: antifungal susceptibility testing, Candida krusei, candidemia, genotyping, short tandem repeats, vaginitis
  • Çukurova Üniversitesi Adresli: Evet

Özet

Candida krusei also known as Pichia kudriavzevii is a potentially multidrug-resistant yeast because it is intrinsically resistant to fluconazole and develops acquired resistance to echinocandins and polyenes. Here, we aim to provide a better understanding of the epidemiology and transmission modes of C. krusei infections by comparing invasive bloodstream (n = 35) and non-invasive vaginal (n = 20) C. krusei isolates. The genetic relatedness of the isolates was assessed using a newly described short tandem repeat (STR) analysis and their sensitivity to eight antifungal compounds was evaluated by antifungal susceptibility testing using the CLSI microbroth dilution method. All C. krusei isolates revealed unique STR genotypes, indicating the absence of clonal transmission in the study group. Furthermore, no drug-resistant or non-wild-type isolates were identified. Our findings demonstrated high resolution of STR genotyping for the detection and simultaneous genetic analysis of multiple C. krusei strains in clinical samples and excellent in vitro activity of common antifungal agents against invasive strains.