Immobilization of pullulanase from Bacillus licheniformis on magnetic multi-walled carbon nanotubes for maltooligosaccharide production


VARAN N. E., ALAGÖZ D., Toprak A., KORKMAZ GÜVENMEZ H., YILDIRIM D.

Chemical Papers, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s11696-024-03764-0
  • Dergi Adı: Chemical Papers
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core
  • Anahtar Kelimeler: Immobilization, Magnetic multi-walled carbon nanotubes, Maltooligosaccharide, Pullulanase
  • Çukurova Üniversitesi Adresli: Evet

Özet

In this study, Fe3O4-coated multi-walled carbon nanotubes (MWCNT-Fe3O4) or nickel oxide-coated multi-walled carbon nanotubes (MWCNT-NiO) were activated with 3-Glycidyloxypropyl)trimethoxysilane (3-GPTMS) to create oxirane groups. Pullulanase from Bacillus licheniformis was covalently immobilized on these magnetic MWCNTs to obtain magnetically separable immobilized pullulanase preparations (MWCNT-Fe3O4@Pul or MWCNT-NiO@Pul) for producing maltooligosaccharides (MOSs) from pullulan. The highest recovered activity values were 78% and 85% respectively, for MWCNT-Fe3O4@Pul and MWCNT-NiO@Pul after 24 h of immobilization at pH 7.0. The optimal pH and temperature were found to be 5.5 and 45 °C for free pullulanase, whereas the corresponding values were 5.5 and 50 °C for both immobilized pullulanase preparations. The thermal stabilities of MWCNT-Fe3O4@Pul and MWCNT-NiO@Pul increased by 6.2- and 8.2-fold, respectively, at 50 °C. The catalytic efficiencies of MWCNT-Fe3O4@Pul and MWCNT-NiO@Pul were calculated to be 0.8- and 1.1-fold that of free pullulanase, respectively. After 24 h of hydrolysis, MOS yields were determined to be 470 and 490 mg MOS/g pullulan for MWCNT-Fe3O4@Pul and MWCNT-NiO@Pul, respectively. The remaining activities were 86% and 85% for MWCNT-Fe3O4@Pul and MWCNT-NiO@Pul after 10 reuses, respectively.