In this study, European sea bass (Dicentrarchus labrax) (20.4) was fed 2%BW for 10 weeks with three different diets containing 0, 50 and 100 of canola oil (Canola, K), as replacement to fish oil. For this purpose, 20 sea bass (78.6 +/- 3.58 grams) (500 L) were stocked in each tank in triplicate. For the determination of the GET, the individual was left fasted for 72 hours before sampling to ensure complete digestion of the digestive tract. Apart from these fasting periods, the individuals were fed "ad libitum". Nine fish were randomly sampled at 0., 8., 16., 24., 32. and 48. hours from each experimental group and the digestive tract were examined in three different sections: the stomach, the anterior and the posterior intestine. The shortest gastric evacuation time (GET) in the stomach, anterior and posterior intestines were found as 32.2, 60.9 and 50.4 h in K0 (0%K) group, while longest as 41.9, 101.3 and 70.2 h in 100-group, respectively. Final data showed that the increase of the ratio of canola used as a substitute to fish oil in the feed, GET was linearly extended in the whole digestive system and this will limit the use of this plant oil source in feed formulations at high rates.