This research was conducted as a main crop in University of Cukurova, Faculty of Agriculture Field Crops Department in 2016 and 2017 growing seasons. The objective of this study was to determine the effect of high air and soil temperature on pod yield and some yield component of peanut (Arachis hypogaea L.) breeding lines in main crop growing season in Mediterranean region in Turkey. In this study, 22 peanut lines (F6 and F7) belonging to Brantley x Halisbey crossing and NC-7 variety (st) were used as a plant material. The experimental design was a Randomized Complete Block with three replications. The main yield component such as pod number, pod weight, 100-seed weight, and pod yield per hectare values of lines were investigated. The maximum air temperature data was recorded day by day during the growing period in both years and average daily maximum air temperatures were higher in 2017 than in 2016. When the pod yield compared in 2016 and 2017, it is found that the pod yield was lower (21.5%) in 2017 than in 2016. The high air and soil temperature was negatively affected the pod yield of peanut breeding lines. It was found that the breeding lines differ in their sensitivity to high temperatures.