9th International Agriculture Congress, Adana, Turkey, 2 - 04 October 2023, pp.187-203
The purpose of this study was to determine the frequency of Rumple disease
in lemon (Citrus lemon L.) growing
regions of the Erdemli district of Mersin Province in Turkiye as well as to
identify citrus viroid agents in the diseased trees. Surveys on the Kütdiken, Meyer,
Aydın, and Interdonato lemon cultivars were carried out in the local lemon
orchards. Rumple disease had a cumulative prevalence rate of 29% in the Erdemli
district's lemon-growing areas. The major symptoms seen in biological indexing
tests were epinasty and leaf necrosis on leaves as a result of mechanical
inoculation on etrog citron seedlings grown from seed. To find out if citrus
exocortis viroid (CEVd) and citrus cachexia viroid (CCaVd) were present in
trees exhibiting collapse symptoms, RT-PCR tests were conducted using samples
from 10 different lemon trees. Following the studies, it was found that 6 of
the samples were infected with CEVd and had a band at a level of 371 bp.
Furthermore, 6 of the tested samples showed CCaVd infection and revealed a band
at the 300 bp level.