Applied Fruit Science, cilt.68, sa.1, 2026 (SCI-Expanded, Scopus)
Citrus production in Mediterranean regions is limited by phosphorus (P) availability due to high lime, pH, and low organic carbon. The present study was conducted under greenhouse conditions with three compost levels (0, 20, and 40 g compost/kg soil), three rock phosphate (RP) levels (0, 2, and 4 g RP/kg soil), and seven mycorrhizal species (control, Glomus mosseae, G. caledonium, G. etunicatum, G. clarium, indigenous mycorrhiza, and a cocktail mixture). Root colonization, shoot and root dry weights, tissue nutrient concentrations, and mycorrhizal dependency (MD) were determined. The combination of arbuscular mycorrhizal (AM) fungi species, rock phosphate, and compost application led to a remarkable increase in dry matter production, root colonization, and nutrient uptake. Higher RP doses tended to suppress colonization of mycorrhizae. An increase in RP application resulted in higher MD across all mycorrhizal species and compost treatments. Mycorrhizal species-inoculated citrus seedlings affected MD. The highest MD (91%) was observed in seedlings inoculated with G. mosseae and treated with 20 g kg−1 compost and 4 g kg−1 RP. The same AM+ compost +RP significantly improved citrus seedling growth and P and zinc (Zn) uptake. The effect of compost on colonization depended on species × dose interactions. Arbuscular mycorrhizal fungi treatments alone increased shoot P concentrations from ~0.04–0.07% in the control and ineffective-local treatments to ~0.08–0.12%, and also increased Zn, iron (Fe), manganese (Mn), and copper (Cu) concentrations. Results showed that selected efficient mycorrhizal spores, when RP is incorporated into compost, can be used for citrus seedling production.