MEDICAL PRINCIPLES AND PRACTICE, vol.14, no.3, pp.189-193, 2005 (SCI-Expanded)
The aim of this study was to determine the reference ranges of lymphocyte subsets in serologically HIV-negative healthy adults in Turkey. Materials and Methods: Blood samples from 220 healthy adults, 105 female and 115 male, collected into tubes containing EDTA were investigated for lymphocyte subsets using flow cytometry. The age range was 18-80 years (44.80 +/- 16.69). Results: The mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 72.70 +/- 8.44%, 1,680 +/- 528 cells/mu l; CD4: 47.37 +/- 9.10%, 1,095 +/- 391 cells/mu l; CD8: 28.99 +/- 5.99%, 669 +/- 239 cells/mu l; CD19: 10.96 +/- 4.44%, 254 +/- 122 cells/mu l and CD56: 7.03 +/- 3.26%, 161 +/- 92 cells/mu l, respectively. The ratio of CD4/CD8 was 1.68 +/- 0.43. There was no statistically significant difference in the percentages and absolute values of lymphocyte subsets between the genders (p > 0.05). Conclusion: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adults in Turkey. Copyright (C) 2005 S. Karger AG, Basel.