Reference ranges of lymphocyte subsets of healthy adults in Turkey

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Yaman A., Cetiner S., Kıbar F., Tasova Y., Seydaoglu G., Dundar I.

MEDICAL PRINCIPLES AND PRACTICE, vol.14, no.3, pp.189-193, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 3
  • Publication Date: 2005
  • Doi Number: 10.1159/000084638
  • Journal Name: MEDICAL PRINCIPLES AND PRACTICE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.189-193
  • Keywords: lymphocyte subsets, healthy adults, flow cytometry, FLOW-CYTOMETRIC ANALYSIS, BLOOD-LYMPHOCYTES, REFERENCE VALUES, WHOLE-BLOOD, AGE, SUBPOPULATIONS, CHILDREN
  • Çukurova University Affiliated: Yes

Abstract

The aim of this study was to determine the reference ranges of lymphocyte subsets in serologically HIV-negative healthy adults in Turkey. Materials and Methods: Blood samples from 220 healthy adults, 105 female and 115 male, collected into tubes containing EDTA were investigated for lymphocyte subsets using flow cytometry. The age range was 18-80 years (44.80 +/- 16.69). Results: The mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 72.70 +/- 8.44%, 1,680 +/- 528 cells/mu l; CD4: 47.37 +/- 9.10%, 1,095 +/- 391 cells/mu l; CD8: 28.99 +/- 5.99%, 669 +/- 239 cells/mu l; CD19: 10.96 +/- 4.44%, 254 +/- 122 cells/mu l and CD56: 7.03 +/- 3.26%, 161 +/- 92 cells/mu l, respectively. The ratio of CD4/CD8 was 1.68 +/- 0.43. There was no statistically significant difference in the percentages and absolute values of lymphocyte subsets between the genders (p > 0.05). Conclusion: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adults in Turkey. Copyright (C) 2005 S. Karger AG, Basel.